Circulating miRNA expression in asthmatics is age-related and associated with clinical asthma parameters, respiratory function and systemic inflammation

Table 4 Univariate and multivariable analysis of association of miRNAs expression with asthma status, age group and age group*asthma status interaction

miRNA	Variable	Univariate analysis		Multivariable analysis
miRNA	Variable	Beta (95% CI)	p-value	Beta (95% CI)	p-value	Model statistics
miRNA-146a	Asthma status	0.01 (− 0.18 to 0.2)	0.92	0.02 (− 0.16 to 0.19)	0.86	R2 = 0.13, R2 Corr. = 0.11, F(3,111) = 5.5, p = 0.002
	Age group	− 0.28 (− 0.46 to − 0.11)	0.002	− 0.28 (− 0.46 to − 0.11)	0.002
	Age group*asthma status	Chi2 = 6.27, p(LR) = 0.01		0.22 (0.05 to 0.4)	0.01
miRNA-126a	Asthma status	− 0.09 (− 0.28 to 0.1)	0.35	− 0.12 (− 0.29 to 0.05)	0.17	R2 = 0.22, R2 Corr. = 0.2, F(3,105) = 9.9, p < 0.001
	Age group	− 0.45 (− 0.62 to − 0.28)	< 0.001	− 0.46 (− 0.63 to - 0.29)	< 0.001
	Age group*asthma status	Chi2 = 0.001. p(LR) = 0.98		0 (− 0.17 to 0.17)	0.98
miRNA-106a	Asthma status	− 0.12 (− 0.3 to 0.06)	0.2	− 0.13 (− 0.3 to 0.05)	0,15	R2 = 0.12, R2 Corr. = 0.09, F(3,111) = 5, p = 0.003
	Age group	− 0.31 (− 0.49 to − 0.14)	< 0.001	− 0.32 (− 0.49 to − 0.14)	< 0.001
	Age group*asthma status	Chi2 = 0.49. p(LR) = 0.49		− 0.06 (− 0.24 to 0.12)	0.49
miRNA-19b	Asthma status	0.17 (− 0.01 to 0.35)	0.07	0.17 (− 0.01 to 0.35)	0.06	R2 = 0.13, R2 Corr. = 0.1, F(3,111) = 5.45, p = 0.002
	Age group	− 0.29 (− 0.47 to − 0.12)	0.001	− 0.29 (− 0.46 to − 0.11)	0.002
	Age group*asthma status	Chi2 = 2.05, p(LR) = 0.15		0.13 (− 0.05 to 0.3)	0.16

In the model, each tested miRNA was used as the dependent variable, while the independent variables were asthma status (asthma vs controls), age group (elderly vs. non-elderly), and age group*asthma status interaction. miRNA expression [2^−ΔCt] was log-2 transformed before the analysis. The bold text indicates a statistically significant p-value

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